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goat anti limp2  (Thermo Fisher)


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    Thermo Fisher goat anti limp2
    Goat Anti Limp2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+limp2/goat+anti+limp+2/pm38666485-505-14-16
    Average 90 stars, based on 1 article reviews
    goat anti limp2 - by Bioz Stars, 2026-09
    90/100 stars

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    Western Blot:

    Article Title: Activation and Purification of ß-Glucocerebrosidase by Exploiting its Transporter LIMP-2 - Implications for Novel Treatment Strategies in Gaucher's and Parkinson's Disease.
    Article Snippet: .. Following antibodies and dilution factors were used for western blot analysis: Primary: goat anti LIMP2 (polyclonal, ThermoFisher Scientific Inc., Waltham, MA, United States, #PA5-19111; dilution: 1:1000), mouse anti GCase (monoclonal, clone E2E, Abnova, Taipeh, Taiwan,#H00002629-M01; dilution: 1:1000), rabbit anti GCase (polyclonal, Sigma-Aldrich, St. Louis, MO, United States, #G4171; dilution: 1:1000), rabbit anti LAMP-2A (monoclonal, clone SA4601, Novus Biologicals, Centennial, CO, United States, #NBP2-67298; dilution: 1:1000), goat anti GAPDH (polyclonal, R&D Systems, Minneapolis, MN, United States, #AF5718; dilution: 1:5000), rabbit anti calnexin (polyclonal, Cell Signaling Technology, Danvers, MA, United States, #2433, dilution: 1:1000). .. Secondary: donkey anti goat Alexa Fluor 680 (ThermoFisher Scientific Inc., Waltham, MA, United States, #A21084; dilution: 1:10 000), IRDye 800CW donkey anti goat (LI-COR Biosciences, Lincoln, NE, United States, #926-32214; dilution: 1:10 000) donkey anti mouse Alexa Fluor 680 (ThermoFisher Scientific Inc., Waltham, MA, United States, #A10038; dilution: 1:10 000), donkey anti rabbit Alexa Fluor 680 (ThermoFisher Scientific Inc., Waltham, MA, United States, #A10043; dilution: 1:10 000).



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    goat anti limp2  (Thermo Fisher)
    90
    Thermo Fisher goat anti limp2
    Goat Anti Limp2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+limp2/goat+anti+limp+2/pm38666485-505-14-16
    Average 90 stars, based on 1 article reviews
    goat anti limp2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    goat anti limp2 (cat# af1966-sp,1:2000)  (Millipore)
    90
    Millipore goat anti limp2 (cat# af1966-sp,1:2000)
    a Experimental workflow for the XL-LC-MS/MS analysis of lysosome-enriched fractions. Created with BioRender.com. b Normalized β-hexosaminidase activities for individual fractions from lysosome enrichment by SPIONs. Data are presented as mean values + SD ( n = 3, biologically independent samples over three independent experiments). c Western blot analysis of lysosome-enriched fractions for contamination by other organelles ( n = 2). Lysosome: lysosomal proteins (CTSD, LAMP2, <t>LIMP2,</t> and LAMTOR1). Other: Golgi apparatus (GM130), cytoskeleton (TUBA), cytosol (GAPDH), endoplasmic reticulum (CANX), and mitochondria (SDHA). d Summed iBAQ abundances for proteins identified in lysosome-enriched fractions in ≥3 replicates. e Classification of unique cross-linked residue pairs. f Proteins detected in non-cross-linked lysosome-enriched fractions (proteome), and unique lysosomal cross-linked residue pairs (interactome) for DR and IT samples. g Localization of CSMs for 68 lysosomal proteins cross-linked in the DR and IT state. Cytosolic: proteins located at the cytosolic face of the lysosomal membrane; Lumen: lysosomal luminal proteins. h Correlation of cross-link identification and protein abundance for lysosomal proteins. CSMs and PSMs represent summed values of the analysis ( n = 6, biologically independent samples over six independent experiments (3× DR and 3× IT)). SPIONs superparamagnetic iron oxide nanoparticles, DR disrupted, IT intact, SCX strong cation-exchange, IN input, FT flow through, W wash, EL eluate, WCL whole-cell lysate, iBAQ intensity-based absolute quantification, XLs cross-links, CSMs cross-link spectral matches, PSMs peptide spectral matches. Source data are provided as a Source Data file.
    Goat Anti Limp2 (Cat# Af1966 Sp,1:2000), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+limp2/goat+anti+limp2/pmc09584938-337-11-29
    Average 90 stars, based on 1 article reviews
    goat anti limp2 (cat# af1966-sp,1:2000) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    goat-anti-limp2  (Millipore)
    90
    Millipore goat-anti-limp2
    Lysosomal integral membrane protein type B <t>(LIMP2)</t> in Npc1 −/− liver and U18666A treated HEPG2 cells; ( A ) Western blot analysis of <t>LIMP2</t> in lysates of Npc1 +/+ and Npc1 −/− mouse liver; ( B ) phase contrast microscopy pictures of HEPG2 cells treated with NPC1-inhibitor U18666A (magnification of 63×); ( C ) GCase in HEPG2 lysates was labelled with GCase-specific ABP and subsequently visualized by fluorescence scanning after SDS-PAGE. LIMP2 was analyzed by Western blotting of the same wet gel slab as described in the Materials and Methods section.
    Goat Anti Limp2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+limp2/goat+anti+limp2/pmc07959463-145-2-6
    Average 90 stars, based on 1 article reviews
    goat-anti-limp2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    a Experimental workflow for the XL-LC-MS/MS analysis of lysosome-enriched fractions. Created with BioRender.com. b Normalized β-hexosaminidase activities for individual fractions from lysosome enrichment by SPIONs. Data are presented as mean values + SD ( n = 3, biologically independent samples over three independent experiments). c Western blot analysis of lysosome-enriched fractions for contamination by other organelles ( n = 2). Lysosome: lysosomal proteins (CTSD, LAMP2, LIMP2, and LAMTOR1). Other: Golgi apparatus (GM130), cytoskeleton (TUBA), cytosol (GAPDH), endoplasmic reticulum (CANX), and mitochondria (SDHA). d Summed iBAQ abundances for proteins identified in lysosome-enriched fractions in ≥3 replicates. e Classification of unique cross-linked residue pairs. f Proteins detected in non-cross-linked lysosome-enriched fractions (proteome), and unique lysosomal cross-linked residue pairs (interactome) for DR and IT samples. g Localization of CSMs for 68 lysosomal proteins cross-linked in the DR and IT state. Cytosolic: proteins located at the cytosolic face of the lysosomal membrane; Lumen: lysosomal luminal proteins. h Correlation of cross-link identification and protein abundance for lysosomal proteins. CSMs and PSMs represent summed values of the analysis ( n = 6, biologically independent samples over six independent experiments (3× DR and 3× IT)). SPIONs superparamagnetic iron oxide nanoparticles, DR disrupted, IT intact, SCX strong cation-exchange, IN input, FT flow through, W wash, EL eluate, WCL whole-cell lysate, iBAQ intensity-based absolute quantification, XLs cross-links, CSMs cross-link spectral matches, PSMs peptide spectral matches. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

    doi: 10.1038/s41467-022-33951-0

    Figure Lengend Snippet: a Experimental workflow for the XL-LC-MS/MS analysis of lysosome-enriched fractions. Created with BioRender.com. b Normalized β-hexosaminidase activities for individual fractions from lysosome enrichment by SPIONs. Data are presented as mean values + SD ( n = 3, biologically independent samples over three independent experiments). c Western blot analysis of lysosome-enriched fractions for contamination by other organelles ( n = 2). Lysosome: lysosomal proteins (CTSD, LAMP2, LIMP2, and LAMTOR1). Other: Golgi apparatus (GM130), cytoskeleton (TUBA), cytosol (GAPDH), endoplasmic reticulum (CANX), and mitochondria (SDHA). d Summed iBAQ abundances for proteins identified in lysosome-enriched fractions in ≥3 replicates. e Classification of unique cross-linked residue pairs. f Proteins detected in non-cross-linked lysosome-enriched fractions (proteome), and unique lysosomal cross-linked residue pairs (interactome) for DR and IT samples. g Localization of CSMs for 68 lysosomal proteins cross-linked in the DR and IT state. Cytosolic: proteins located at the cytosolic face of the lysosomal membrane; Lumen: lysosomal luminal proteins. h Correlation of cross-link identification and protein abundance for lysosomal proteins. CSMs and PSMs represent summed values of the analysis ( n = 6, biologically independent samples over six independent experiments (3× DR and 3× IT)). SPIONs superparamagnetic iron oxide nanoparticles, DR disrupted, IT intact, SCX strong cation-exchange, IN input, FT flow through, W wash, EL eluate, WCL whole-cell lysate, iBAQ intensity-based absolute quantification, XLs cross-links, CSMs cross-link spectral matches, PSMs peptide spectral matches. Source data are provided as a Source Data file.

    Article Snippet: The following primary antibodies were used in this study: goat anti LIMP2 (Cat# AF1966-SP,1:2000), mouse anti ACT2 (Cat# A5316, 1:4000), and rabbit anti LAMTOR1 (Cat # HPA002997, 1:1000) from Sigma-Aldrich; mouse anti CANX (Cat# 66903-1-AP, 1:20,000), mouse anti FLOT2 (Cat# 66881-1-Ig, 1:1500), mouse anti FZD9 (Cat# 67023-1-Ig, 1:1500), rabbit anti ATP6V1D (Cat# 14920-1-AP, 1:1000), rabbit anti CTSD (Cat# # 21327-1-AP, 1:1000), rabbit anti FLOT1 (Cat# 15571-1-AP, 1:2000), rabbit anti GNB4 (Cat# 11978-2-AP, 1:2000), and rabbit anti SDHA (Cat# 14865-1-AP, 1:800) from Proteintech; goat anti LIMP2 (Cat# AF1966-SP, 1:2000) from R&D system; mouse anti ATP6V1B2 (Cat# SC166045, 1:1000) from Santa Cruz; mouse anti FLOT1 (Cat# 610821, 1:200), mouse anti FLOT2 (Cat# 610383, 1:200), and mouse anti GM130 (Cat# 610822) from BD Biosciences; mouse anti GAPDH (Cat# 5174, 1:2500), rabbit anti EEA1 (Cat# 2411, 1:200), rabbit anti FLOT1 (Cat# 18634, 1:200), and rabbit anti RRAGA (Cat# 4357, 1:1000) from Cell signaling; rabbit anti LPHN2 (Cat# NBP2-58704, 1:100) and rabbit anti LPHN3 (Cat# NLS1138, 1:200) from Novus Biologicals; goat anti-mouse IgG (H + L)-Alexa Fluor 488 (Cat# A-11029, 1:400), rabbit anti ATP6V1A1 (Cat# PA5-29191,1:2000), and rabbit anti LPHN1 (Cat # PA5-77475, 1:200) from Thermo Fisher Scientific; rabbit anti TUBA (Cat# 600-401-880, 1:2000) from Rockland; mouse anti LAMP2 (Cat# H4B4, 1:1000 for WB, 1:100 for IF) from Hybridoma Bank. rabbit anti DSSO (Self-made , 1:5000).

    Techniques: Liquid Chromatography with Mass Spectroscopy, Western Blot, Residue, Membrane

    Lysosomal integral membrane protein type B (LIMP2) in Npc1 −/− liver and U18666A treated HEPG2 cells; ( A ) Western blot analysis of LIMP2 in lysates of Npc1 +/+ and Npc1 −/− mouse liver; ( B ) phase contrast microscopy pictures of HEPG2 cells treated with NPC1-inhibitor U18666A (magnification of 63×); ( C ) GCase in HEPG2 lysates was labelled with GCase-specific ABP and subsequently visualized by fluorescence scanning after SDS-PAGE. LIMP2 was analyzed by Western blotting of the same wet gel slab as described in the Materials and Methods section.

    Journal: International Journal of Molecular Sciences

    Article Title: GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice

    doi: 10.3390/ijms22052532

    Figure Lengend Snippet: Lysosomal integral membrane protein type B (LIMP2) in Npc1 −/− liver and U18666A treated HEPG2 cells; ( A ) Western blot analysis of LIMP2 in lysates of Npc1 +/+ and Npc1 −/− mouse liver; ( B ) phase contrast microscopy pictures of HEPG2 cells treated with NPC1-inhibitor U18666A (magnification of 63×); ( C ) GCase in HEPG2 lysates was labelled with GCase-specific ABP and subsequently visualized by fluorescence scanning after SDS-PAGE. LIMP2 was analyzed by Western blotting of the same wet gel slab as described in the Materials and Methods section.

    Article Snippet: For , goat-anti-LIMP2 was used (SAB2501242, Sigma).

    Techniques: Western Blot, Microscopy, Fluorescence, SDS Page

    Immunohistochemistry NPC liver; “Composite” panels of immunostaining of Npc1 +/+ and Npc1 −/− liver of 80-day-old mice. The first three panels show IBA1, cathepsin D, and LAMP1 in yellow and LIMP2 in magenta. The last panel shows LAMP1 in yellow and cathepsin D in magenta. Brightfield scans were analyzed using spectral imaging; separate images are displayed in heat-map intensity scale. Scale bar = 50 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice

    doi: 10.3390/ijms22052532

    Figure Lengend Snippet: Immunohistochemistry NPC liver; “Composite” panels of immunostaining of Npc1 +/+ and Npc1 −/− liver of 80-day-old mice. The first three panels show IBA1, cathepsin D, and LAMP1 in yellow and LIMP2 in magenta. The last panel shows LAMP1 in yellow and cathepsin D in magenta. Brightfield scans were analyzed using spectral imaging; separate images are displayed in heat-map intensity scale. Scale bar = 50 µm.

    Article Snippet: For , goat-anti-LIMP2 was used (SAB2501242, Sigma).

    Techniques: Immunohistochemistry, Immunostaining, Imaging

    Immunofluorescence of LIMP2 and bile salt efflux pump (BSEP) in hepatocytes of Npc1 +/+ and Npc1 −/− liver. Scale bar = 20 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice

    doi: 10.3390/ijms22052532

    Figure Lengend Snippet: Immunofluorescence of LIMP2 and bile salt efflux pump (BSEP) in hepatocytes of Npc1 +/+ and Npc1 −/− liver. Scale bar = 20 µm.

    Article Snippet: For , goat-anti-LIMP2 was used (SAB2501242, Sigma).

    Techniques: Immunofluorescence